新疆牛源产志贺毒素大肠杆菌耐药性及其ESBLs基因鉴定

doi:10.11843/j.issn.0366-6964.2019.04.022

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (4): 887-892.doi: 10.11843/j.issn.0366-6964.2019.04.022

新疆牛源产志贺毒素大肠杆菌耐药性及其ESBLs基因鉴定

佟盼盼, 马凯琪, 刘争辉, 谢金鑫, 苏红, 王栋, 孙雪, 高姣姣, 苏战强*

新疆农业大学动物医学学院, 乌鲁木齐 830052

收稿日期:2018-10-11 出版日期:2019-04-23 发布日期:2019-04-23
通讯作者: 苏战强,主要从事动物源食品安全防控技术研究,E-mail:szq00009@163.com
作者简介:佟盼盼(1986-),女,吉林松原人,博士,讲师,主要从事细菌耐药性传播机制研究,E-mail:tongpanpan123@163.com;马凯琪(1995-),女,新疆乌鲁木齐人,硕士生,主要从事动物病原菌的耐药性研究,E-mail:1650640471@qq.com
基金资助:
新疆农业大学校前期资助课题（XJAU201703）；新疆农业大学研究生科研创新项目（XJAUGRI2017-010）；新疆农业大学国家级大学生创新创业训练计划项目（201810758011）

Characterization of Antimicrobial Resistance and Extended-Spectrum β-Lactamase Genes in Bovine Origin Shiga Toxin-producing Escherichia coli in Xinjiang

TONG Panpan, MA Kaiqi, LIU Zhenghui, XIE Jinxin, SU Hong, WANG Dong, SUN Xue, GAO Jiaojiao, SU Zhanqiang*

College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China

Received:2018-10-11 Online:2019-04-23 Published:2019-04-23

摘要/Abstract

摘要：

本研究的目的是通过调查新疆地区分离的牛源产志贺毒素大肠杆菌（STEC）的耐药表型和基因型，掌握STEC耐药性的发展和传播规律。本研究对新疆6个地区的牛源（非O157：H7）STEC分离株进行了18种抗生素的药物敏感性试验，并检测菌株中携带的超广谱β-内酰胺酶（ESBLs）基因。结果显示：4.31% STEC表现为多重耐药，1.91%为产ESBLs菌株。检测到的主要ESBLs基因包括bla_TEM和bla_CTX-M。这是首次在新疆STEC中检测到bla_TEM和bla_CTX-M。本研究分离出的多数多重耐药STEC属于系统发育A群。多重耐药STEC可能是由非致病性大肠杆菌获得毒性和耐药基因而形成的。抗生素的选择压力可能对细菌在牛肠道中的定植表现出一定竞争优势，从而增加了耐药STEC对食物的污染。

Abstract:

The objective of this study was to investigate the phenotypic and genotypic basis of bovine Shiga toxin-producing Escherichia coli (STEC) from Xinjiang and obtain the evolution and spread of STECs resistance. Susceptibility testing to 18 antimicrobials was performed on bovine (non-O157:H7)STEC isolates from 6 regions of Xinjiang. Meanwhile, the extended spectrum β-lactamases(ESBL) genes were amplified. In this study 4.31% STEC isolates were multidrug resistant to antimicrobials (MDR), and 1.91% were ESBLs-producing strains. The predominant ESBL genes detected were bla_TEM and bla_CTX-M. This is the first report of bla_TEM and bla_CTX-M in STEC isolates in Xinjiang. Most resistant STECs (91.67%) isolated in this study belong to phylogenetic groups A. These findings suggest that MDR STECs are emerging as a result of nonpathogenic E. coli acquiring virulence and resistance genes. This may convey a certain competitive advantage for the colonization of these STECs in cattle when antimicrobial selective pressures are present, potentially leading to an increase in contamination of food with resistant STECs.

中图分类号:

佟盼盼, 马凯琪, 刘争辉, 谢金鑫, 苏红, 王栋, 孙雪, 高姣姣, 苏战强. 新疆牛源产志贺毒素大肠杆菌耐药性及其ESBLs基因鉴定[J]. 畜牧兽医学报, 2019, 50(4): 887-892.

TONG Panpan, MA Kaiqi, LIU Zhenghui, XIE Jinxin, SU Hong, WANG Dong, SUN Xue, GAO Jiaojiao, SU Zhanqiang. Characterization of Antimicrobial Resistance and Extended-Spectrum β-Lactamase Genes in Bovine Origin Shiga Toxin-producing Escherichia coli in Xinjiang[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 887-892.

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新疆牛源产志贺毒素大肠杆菌耐药性及其ESBLs基因鉴定

Characterization of Antimicrobial Resistance and Extended-Spectrum β-Lactamase Genes in Bovine Origin Shiga Toxin-producing Escherichia coli in Xinjiang

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